Peroxidase (orisun Horseradish) Itumọ: Hydrogen peroxide oxidoreductase;HRP

Horseradish peroxidase (HRP) ti ya sọtọ lati awọn gbongbo ti horseradish (Amoracia rusticana) ati pe o jẹ ti ẹgbẹ ferroprotoporphyrin ti peroxidases.HRP ni imurasilẹ daapọ pẹlu hydrogen peroxide (H2O2).Abajade [HRP-H2O2] eka le oxidize ọpọlọpọ awọn oluranlọwọ hydrogen:
Oluranlọwọ + H2O2 → Oluranlọwọ Oxidized + 2 H2O
HRP yoo oxidize orisirisi awọn sobusitireti (wo Tabili 1):
• Chromogenic
• Chemiluminescent (gẹgẹbi luminol tabi isoluminol)
• Fluorogenic (bii tyramine, homovanillic acid, tabi 4-hydroxyphenyl acetic acid)
HRP jẹ polypeptide pq kan ti o ni awọn afara disulfide mẹrin.HRP jẹ glycoprotein ti o ni 18% carbohydrate ninu.Akopọ carbohydrate ni galactose, arabinose, xylose, fucose, mannose, mannosamine, ati galactosamine, da lori isozyme kan pato.

HRP jẹ aami ti a lo pupọ fun immunoglobulins ni ọpọlọpọ awọn ohun elo ajẹsara ti o yatọ, pẹlu immunoblotting, immunohistochemistry, ati ELISA.HRP le ṣe idapọmọra si awọn apo-ara nipasẹ ọpọlọpọ awọn ọna oriṣiriṣi, pẹlu glutaraldehyde, oxidation periodate, nipasẹ awọn ifunmọ disulfide, ati paapaa nipasẹ amino ati thiol ti o darí awọn ọna asopọ agbelebu.HRP jẹ aami ti o fẹ julọ fun awọn aporo-ara, nitori pe o kere julọ ati iduroṣinṣin julọ ti awọn aami enzymu olokiki mẹta julọ (peroxidase, β-galactosidase, alkaline phosphatase) ati glycosylation rẹ nyorisi isunmọ isunmọ ti kii ṣe pato.Atunyẹwo ti glutaraldehyde ati awọn ọna isọpọ akoko ti jẹ atẹjade.

A tun lo Peroxidase fun ipinnu ti glukosi4 ati peroxides ni ojutu.Ọpọlọpọ awọn atẹjade, 6-24 theses, 25-29 ati awọn iwe afọwọkọ 30-46 ti tọka si lilo P8375 ninu awọn ilana iwadii wọn.