Alkaline Phosphatase (ALP)

Cas No.: 9001-78-9

Mimọ.: 90%

Package: 100μL, 500μL, 10ml, 100ml, 1000ml

Alaye ọja

Apejuwe

Alkaline Phosphatase ti wa lati inu igara E. coli atunko ti o gbe jiini TAB5.Enzymu naa ṣe itọsi dephosphorylation ti 5 'ati 3' opin DNA ati awọn phosphomonoesters RNA.Bakannaa, o ṣe hydrolyses ribose, bakanna bi deoxyribonucleoside triphosphates (NTPs ati dNTPs).TAB5 Alkaline Phosphatase ṣiṣẹ lori 5 'protruding, 5' recessed ati blunt pari.Phosphatase le ṣee lo ni ọpọlọpọ awọn ohun elo isedale molikula, gẹgẹbi cloning tabi isamisi ipari iwadii lati yọ awọn opin phosphorylated ti DNA tabi RNA kuro.Ninu awọn adanwo ti cloning, dephosphorylation ṣe idiwọ DNA plasmid laini lati isọ-ara-ẹni.O tun le dinku awọn dNTP ti ko ni idapọ ninu awọn aati PCR lati mura awoṣe kan fun titẹle DNA.Enzymu naa jẹ ailagbara patapata ati aibikita nipasẹ alapapo ni 70°C fun awọn iṣẹju 5, nitorinaa ṣiṣe yiyọ phosphatase kuro ṣaaju iṣọtẹ tabi ifamisi ipari ko ṣe pataki.

Kemikali Be

Sipesifikesonu

Awọn nkan Idanwo	Awọn pato
Iṣẹ iṣe enzymu	5U/μL
Mimo	95%
Iṣẹ-ṣiṣe Endonuclease	Ko ṣee wa-ri
Iṣẹ-ṣiṣe Exonuclease	Ko ṣee wa-ri
Iṣẹ ṣiṣe Nicking	Ko ṣee wa-ri
Iṣẹ RNase	Ko ṣee wa-ri
E.coli DNA	≤1 ẹda/5U
Endotoxin	Idanwo LAL, ≤ 10EU/mg