Bst 2.0 DNA Polymerase (ọfẹ glycerol, iwuwo giga)
Bst DNA polymerase V2 wa lati Bacillus stearothermophilus DNA Polymerase I, eyiti o ni iṣẹ 5′→3′ DNA polymerase ati iṣẹ rirọpo pq ti o lagbara, ṣugbọn ko si iṣẹ-ṣiṣe 5′→3′ exonuclease.Bst DNA Polymerase V2 jẹ apere fun gbigbe-nipo okun, isothermal ampilifaya LAMP (Afikun isothermal mediated Loop) ati itọsẹ kiakia.Bst DNA polymerase V2 yii ni o lagbara lati ṣe idiwọ iṣẹ ṣiṣe DNA polymerase ni iwọn otutu yara, ki o le ṣiṣẹ ati eto ifakalẹ le ṣe agbekalẹ ni iwọn otutu yara, ṣe idiwọ imudara ti kii ṣe pato ati imudara ṣiṣe ti iṣesi, ati pe ẹya yii le jẹ lyophilized.Ni afikun, o lagbara lati ṣe idasilẹ iṣẹ rẹ ni awọn iwọn otutu ti o ga, nitorinaa imukuro iwulo fun igbesẹ imuṣiṣẹ lọtọ.
Awọn eroja
| Ẹya ara ẹrọ | HC5007A-01 | HC5007A-02 | HC5007A-03 |
| Bst DNA polymerase V2 (ọfẹ glycerol) (32U/μL) | 0,05 milimita | 0,25 milimita | 2.5 milimita |
| 10×HC Bst V2 saarin | 1,5 milimita | 2×1.5 milimita | 3×10 milimita |
| MgSO4 (100mM) | 1,5 milimita | 2×1.5 milimita | 2×10 milimita |
Awọn ohun elo
1.Imudara isothermal LAMP
2.Okun DNA nikan nipo nipo
3.Ga GC jiini lesese
4.Ilana DNA ti ipele nanogram.
Ibi ipamọ Ipo
Gbigbe labẹ 0°C ati ki o wa ni ipamọ ni -25°C~-15°C.
Unit Definition
Ẹyọ kan jẹ asọye bi iye henensiamu ti o ṣafikun 25 nmol ti dNTP sinu ohun elo airotẹlẹ acid ni ọgbọn iṣẹju ni 65°C.
Atupa lenu
| Awọn eroja | 25 μLEto |
| 10×HC Bst V2 saarin | 2.5 μL |
| MgSO4 (100mM) | 1.5 μL |
| dNTPs (10mM kọọkan) | 3.5 μL |
| SYTO™ 16 Alawọ ewe (25×)a | 1.0 μL |
| Alakoko illab | 6 μL |
| Bst DNA Polymerase V2 (Glycerol-ọfẹ) (32 U/ul) | 0.25 μL |
| Àdàkọ | × μL |
| ddH₂O | Titi di 25 μl |
Awọn akọsilẹ:
1) a.SYTOTM 16 Green (25×): Gẹgẹbi awọn iwulo idanwo, awọn awọ miiran le ṣee lo bi aropo;
2) b.Ijọpọ alakoko: ti a gba nipasẹ didapọ 20µM FIP, 20 µM BIP, 2.5 µM F3, 2.5 µM B3, 5 µM LF, 5 µM LB ati awọn ipele miiran.
Ifesi ati ipo
1 × HC Bst V2 Buffer, iwọn otutu idawọle wa laarin 60°C ati 65°C.
Ooru Inactivation
80 °C, 20 iṣẹju.
