Fluorisenti RT-LAMP (bọọlu Lyophilized)
RT-LAMP Fluorescent Master Mix (Lyophilized Powder) ni ifasimu ifasilẹ, Bst DNA polymerase ati ooru-sooro yiyipada transcriptase, aabo lyophilized, ati awọ fluorescent.Ọja naa wa ni irisi awọn ilẹkẹ lyophilized, ati pe awọn alakoko ati awọn awoṣe nikan nilo lati ṣafikun fun lilo.
Apakan Reagent
RT-LAMP Fluorescent Master Mix (Lyophilized Powder)
Lilo
Fun DNA tabi RNA isothermal ampilifaya
Awọn ipo ipamọ
Gbigbe ati fipamọ ni 2 ~ 8 ° C.
Selifu aye: 12 osu.
Ilana
1. Mu nọmba ti o baamu ti awọn ilẹkẹ lyophilized jade ni ibamu si nọmba awọn idanwo.
2. Ifaseyin eto igbaradi
| Ẹya ara ẹrọ | Iwọn lilo |
| RT-LAMP Fuluorisenti Titunto Mix (Lọlu ti o wa ni Lyophilized) | 2 awọn ilẹkẹ |
| Awọ Fuluorisenti | x μL |
| 10 × Alakoko Mix a | 5 μL |
| Awọn awoṣe DNA/RNA b | n μL |
| Lapapọ | 50 μL |
Awọn akọsilẹ:
1) a.10× Alakoko Mix : 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;
2) b.Awọn awoṣe nucleic acid ni a ṣe iṣeduro lati tuka ni omi DEPC.
Hyasen Biotechnology Co., Ltd
3) eto ifaseyin
| Iwọn otutu | Aago | Awọn iyipo |
| 65℃ | 60-orundun a | 30 |
Awọn akọsilẹ:
1. Gba Fuluorisenti ami
Awọn akọsilẹ
1. Awọn iwọn otutu lenu le jẹ iṣapeye laarin 62°C ati 68°C ni ibamu si alakoko.awọn ipo;
2. Awọn ṣàdánwò yẹ ki o standardize awọn isẹ, pẹlu awọn igbaradi ti awọn lenu eto,ayẹwo processing ati dosing;
3. Lati yago fun idoti, o gba ọ niyanju lati ṣeto eto ifaseyin ni tabili mimọ-olekenkaki o si fi awọn awoṣe kun iho èéfín ni awọn yara miiran lati yago fun kikọlu rere eke.
