Fructosyl-peptide Oxidase (FPOX)
Apejuwe
Enzymu wulo fun ipinnu fructosyl-peptide ati fructosyl-L-amino acid.
Kemikali Be
Ilana Ifa
Fructosyl-peptide + H2O + O2→ Peptide + Glucosone + H2O2
Sipesifikesonu
| Awọn nkan Idanwo | Awọn pato |
| Apejuwe | Lulú amorphous funfun, lyophilized |
| Iṣẹ-ṣiṣe | ≥4U/mg |
| Mimo(SDS-PAGE) | ≥90% |
| Catalase | ≤0.01% |
| ATPase | ≤0.005% |
| Glukosi oxidase | ≤0.03% |
| Cholesterol oxidase | ≤0.003% |
Gbigbe ati ibi ipamọ
Gbigbe: Ibaramu
Ibi ipamọ:Tọju ni -20°C(igba pipẹ), 2-8°C (akoko kukuru)
Niyanju tun-idanwoIgbesi aye:2 odun
Itan idagbasoke
Ọkan ninu awọn atọka ti a lo ninu awọn iwadii aisan ti àtọgbẹ jẹ haemoglobin glycated (HbA1c).Iwọn HbA1c nipa lilo awọn enzymu dara fun sisẹ awọn nọmba nla ti awọn apẹẹrẹ, ati pe o jẹ idiyele daradara.Bii iru bẹẹ, ipe ti o lagbara ti pẹ lati ọdọ awọn oṣiṣẹ ilera fun idagbasoke iru igbelewọn enzymu kan.Nitorinaa, a ṣe agbekalẹ idanwo tuntun nipa lilo “ọna dipeptide”.Ni pato, a ṣe awari “Fructosyl-peptide Oxidase” (FPOX) eyiti o le ṣee lo bi enzymu fun idanwo yii.Eyi jẹ ki a ṣaṣeyọri aṣeyọri akọkọ ni agbaye nipasẹ ṣiṣe otitọ ti idanwo enzymu HbA1c kan.“Ọna dipeptide” yii nlo Protease (enzymu Proteolytic) lati fọ HbA1c ninu ẹjẹ, ati lẹhinna ṣe iwọn awọn ipele ti awọn dipeptides saccharified ti a ṣe ni lilo FPOX.Ọna yii pade pẹlu gbigba idaniloju to lagbara nitori awọn iteriba rẹ ti jijẹ rọrun, ilamẹjọ ati iyara, ati pe HbA1c wiwọn reagent lilo FPOX ti wa ni lilo ni gbogbo agbaye.
