Alkaline Phosphatase (ALP)

Cas No.: 9001-78-9

EC No.: 3.1.3.1

Package: 100μL, 500μL, 10ml, 100ml, 1000ml

ọja Apejuwe

Apejuwe

Alkaline Phosphatase ti wa lati inu igara E. coli atunko ti o gbe jiini TAB5.Enzymu n ṣe itọsi dephosphorylation ti 5 'ati 3' opin DNA ati awọn phosphomonoesters RNA.Pẹlupẹlu, o ṣe hydrolyses ribose, bakanna bi awọn triphosphates deoxyribonucleoside (NTPs ati dNTPs).TAB5 Alkaline Phosphatase ṣiṣẹ lori 5 'protruding, 5' recessed ati blunt pari.Phosphatase le ṣee lo ni ọpọlọpọ awọn ohun elo isedale molikula, gẹgẹbi ti cloning tabi isamisi ipari iwadii lati yọ awọn opin phosphorylated ti DNA tabi RNA kuro.Ninu awọn adanwo ti cloning, dephosphorylation ṣe idilọwọ DNA plasmid laini lati isọ-ara-ẹni.O tun le ba awọn dNTP ti a ko dapọ silẹ ni awọn aati PCR lati mura awoṣe fun tito lẹsẹsẹ DNA.Enzymu naa jẹ ailagbara patapata ati aibikita nipasẹ alapapo ni 70 ° C fun awọn iṣẹju 5, nitorinaa ṣiṣe yiyọ kuro ti phosphatase ṣaaju iṣọpọ tabi ipari isamisi ko ṣe pataki.

Lilo

1.Alkaline phosphatase pọ si awọn ọlọjẹ (awọn egboogi, streptavidin ati be be lo,) le ṣe idanimọ awọn ohun elo ti o wa ni pato, ati pe o le ṣee lo ni ELISA, WB ati wiwa histochemical;
2.Alkaline phosphatase le ṣee lo lati dephosphorize awọn 5 '-terminal ti DNA tabi RNA lati se ara-sisopọ;
3.Dephosphorylated DNA tabi RNA ti o wa loke le jẹ aami nipasẹ awọn fosifeti ti o ni aami redio (nipasẹ T4 poly-nucleotide kinase)

Kemikali Be

Sipesifikesonu

Awọn nkan Idanwo	Awọn pato
Iṣẹ iṣe enzymu	5U/μL
Iṣẹ-ṣiṣe Endonuclease	Ko ri
Iṣẹ Exonuuclease	Ko ri
Iṣẹ ṣiṣe Nicking	Ko ri
Iṣẹ RNase	Ko ri
E.coli DNA	≤1 ẹda/5U
Endotoxin	Idanwo LAL, ≤10EU/mg
Mimo	≥95%