D-Lactate dehydrogenase (LDH)

Cas No.: 9001-60-9

EC No.: 1.1.1.27

Package: 10ku,100ku, 500ku, 1000ku

Alaye ọja

Apejuwe

A lo enzymu fun yiyọ pyruvate kuro ni awọn ipinnu ṣiṣẹ pẹlu NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).

Lilo

1.Alkaline phosphatase pelu awọn ọlọjẹ (egboogi, streptavidin ati be be lo,) le ṣe idanimọ awọn ohun elo ti o fojusi ni pato, ati pe o le ṣee lo ni ELISA, WB ati wiwa histochemical;
2.Alkaline phosphatase le ṣee lo lati dephosphorize awọn 5 '-terminal ti DNA tabi RNA lati se ara-sisopọ;
3.Dephosphorylated DNA tabi RNA ti o wa loke le jẹ aami nipasẹ awọn fosifeti ti o ni aami redio (nipasẹ T4 poly-nucleotide kinase)

Kemikali Be

Ilana ifaseyin

D-Lactate + NAD⁺→ Pyruvate +NADH + H⁺

Sipesifikesonu

Awọn nkan Idanwo	Awọn pato
Apejuwe	Lulú amorphous funfun, lyophilized
Iṣẹ-ṣiṣe	≥200U/mg
Mimo(SDS-iwe)	≥90%
Solubility (10mg lulú/ml)	Ko o
NADH/NADPH oxidase	≤0.01%
Malate dehydrogenase	≤0.005%
Glutamate dehydrogenase	≤0.003%
Pyruvate kinase	≤0.03%
Glutamate Dehydrogenase	≤0.003%
Aspartate aminotransferase	≤0.001%
Alamine aminotransferase	≤0.001%