Glukosi-6-fosifeti dehydrogenase (G6PDH)
Apejuwe
Glucose 6 phosphate dehydrogenase (G6PD) aipe jẹ ipo ajogun ninu eyiti awọn sẹẹli ẹjẹ pupa fọ lulẹ (hemolysis) nigbati ara ba farahan si awọn ounjẹ kan, awọn oogun, awọn akoran tabi wahala.O waye nigbati eniyan ba nsọnu tabi ni awọn ipele kekere ti enzyme glucose-6-phosphate dehydrogenase.Enzymu yii ṣe iranlọwọ fun awọn sẹẹli ẹjẹ pupa ṣiṣẹ daradara.Awọn aami aisan lakoko iṣẹlẹ hemolytic le pẹlu ito dudu, rirẹ, paleness, iyara ọkan iyara, kuru ẹmi, ati awọ ofeefee si (jaundice).Aipe G6PD ni a jogun ni ọna ipadasẹhin ti o ni asopọ X ati pe awọn aami aisan jẹ wọpọ julọ ni awọn ọkunrin (paapaa awọn ọmọ Amẹrika Amẹrika ati awọn ti o wa lati awọn apakan kan ti Afirika, Esia, ati Mẹditarenia).O ṣẹlẹ nipasẹ awọn iyipada jiini ninu jiini G6PD.
Glucose-6-Phophate Dehydrogenase (G-6-PDH) ni awọn ipin meji ti iwuwo molikula deede. Ilana amino acid ti monomer ni a ti gbejade.G-6-PDH ti lo ni awọn idanwo fun nicotinamide adenine dinucleotide ati tissue pyridine nucleotides.G-6-PDH le tun mu ṣiṣẹ lati awọn ojutu urea-denatured.
Glucose 6-phosphate dehydrogenase jẹ enzymu ilana ilana bọtini ni igbesẹ akọkọ ti ọna fosifeti pentose.G-6-P-DH oxidizes glucose-6- fosifeti ni iwaju NADP+ lati so 6-phosphogluconate.Polyacrylamide gel electrophoresis, abawọn iṣẹ-ṣiṣe, ati egboogi-iwukara G-6- PDH antibody immunoblotting awọn iwadi ti fihan pe G-6-PDH jẹ glycoprotein.
Kemikali Be
Ilana Ifa
D-glukosi-6-fosifeti + NAD+→D-Glucono-δ-lactone-6-fosifeti + NADH+H+
Sipesifikesonu
| Awọn nkan Idanwo | Awọn pato |
| Apejuwe | Lulú amorphous funfun, lyophilized |
| Iṣẹ-ṣiṣe | ≥150U/mg |
| Mimo(SDS-iwe) | ≥90% |
| Solubility (10mg lulú / milimita) | Ko o |
| NADH/NADPH oxidase | ≤0.1% |
| Glutathione reductase | ≤0.001% |
| phosphoglucose isomerase | ≤0.001% |
| Creatine phosphokinase | ≤0.001% |
| 6-Phosphogluconate dehydrogenase | ≤0.01% |
| Myokinase | ≤0.01% |
| Hexokinase | ≤0.001% |
Gbigbe ati ibi ipamọ
Gbigbe: Ibaramu
Ibi ipamọ:Fipamọ ni 2-8 ° C
Niyanju tun-idanwoIgbesi aye:2 odun
