Hexokinase (HK)
Apejuwe
Lo Hexokinase fun ipinnu D-glucose, D-fructose, ati D-sorbitol ninu ounjẹ tabi awọn ayẹwo iwadii ti ibi.Enzymu naa tun lo fun idanwo ti awọn saccharide miiran ti o jẹ iyipada si glukosi tabi fructose, ati pe o wulo ni idanwo ti ọpọlọpọ awọn glycosides.
Ti a ba lo Hexokinase ni apapo pẹlu glucose-6-phosphate dehydrogenase (G6P-DH) * (awọn ayẹwo glucose6-phosphate ti a ṣẹda nipasẹ Hexokinase), awọn ayẹwo ko yẹ ki o jẹ ti awọn ifọkansi fosifeti giga bi G6P-DH ti ni idinamọ ni idije nipasẹ fosifeti.
Kemikali Be
Ilana Ifa
D-Hexose + ATP --Mg2+→ D-Hexose-6-fosifeti + ADP
Sipesifikesonu
| Awọn nkan Idanwo | Awọn pato |
| Apejuwe | Funfun si kekere ofeefee amorphous lulú, lyophilized |
| Iṣẹ-ṣiṣe | ≥30U/mg |
| Mimo(SDS-PAGE) | ≥90% |
| Solubility (10mg lulú / milimita) | Ko o |
| Awọn ọlọjẹ | ≤0.01% |
| ATPase | ≤0.03% |
| phosphoglucose isomerase | ≤0.001% |
| Creatine phosphokinase | ≤0.001% |
| Glukosi-6-Phosphate dehydrogenase | ≤0.01% |
| NADH/NADPH oxidase | ≤0.01% |
Gbigbe ati ibi ipamọ
Gbigbe: Aibaramu
Ibi ipamọ:Tọju ni -20°C(igba pipẹ), 2-8°C (akoko kukuru)
Niyanju tun-idanwoIgbesi aye:1 odun
jẹmọ awọn ọja
Kọ ifiranṣẹ rẹ nibi ki o si fi si wa
