Cholesterol oxidase (COD/CHOD)
Apejuwe
Cholesterol oxidase (CHOD) ṣe itọsi igbesẹ akọkọ ninu catabolism idaabobo awọ.Diẹ ninu awọn kokoro arun ti kii ṣe pathogenic, gẹgẹbi Streptomyces ni anfani lati lo idaabobo awọ bi orisun erogba.Awọn kokoro arun pathogenic, gẹgẹbi Rhodococcus equi, nilo CHOD lati ṣe akoran macrophage ogun kan.CHOD jẹ iṣẹ-ṣiṣe.Cholest-5-en-3-one ti wa ni isomerized si cholest-4-en3-one. Idahun isomerization le jẹ iyipada ni apakan.Iṣẹ ṣiṣe ti CHOD da lori awọn ohun-ini ti ara ti awo ilu si eyiti a so sobusitireti naa.
A lo CHOD lati pinnu idaabobo awọ ara.O jẹ enzymu keji ti a lo pupọ julọ ni awọn ohun elo iwadii lẹhin glucose oxidase.CHOD tun wa ohun elo ni microanalysis ti awọn sitẹriọdu ni awọn ayẹwo ounje ati ni iyatọ 3-ketosteroids lati 3b-hydroxysteroids. Awọn ohun elo transgenic ti n ṣalaye idaabobo awọ oxidase ti wa ni iwadi ni igbejako owu boll owu.Cholesterol oxidase tun ti lo bi iwadii molikula lati ṣe alaye awọn ẹya awọ ara cellular.
Kemikali Be
Ilana Ifa
Cholesterol + O2 →△4-Cholesten-3-ọkan + H2O2
Sipesifikesonu
| Awọn nkan Idanwo | Awọn pato |
| Apejuwe | Lulú amorphous Yellowish, lyophilized |
| Iṣẹ-ṣiṣe | ≥8U/mg |
| Mimo(SDS-iwe) | ≥90% |
| Solubility (10mg lulú / milimita) | Ko o |
| Catalase | ≤0.001% |
| Glukosi oxidase | ≤0.01% |
| Cholesterol esterase | ≤0.01% |
| ATPase | ≤0.005% |
Gbigbe ati ibi ipamọ
Gbigbe:Ti firanṣẹ labẹ -15 ° C
Ibi ipamọ:Tọju ni -25~-15°C(igba pipẹ), 2-8°C(igba kukuru)
Niyanju tun-idanwoIgbesi aye:1 odun
