Ultra Nuclease
UltraNuclease jẹ jiini engineedendonuclaese ti o wa lati Serratia marcescens, eyiti o lagbara lati dinku DNA tabi RNA, boya ilọpo meji tabi okun ẹyọkan, laini tabi ipin labẹ ọpọlọpọ awọn ipo, dinku awọn acids nucleic patapata sinu 5'-monophosphate oligonucleotides pẹlu ipari gigun 3-5base. .Lẹhin iyipada ti imọ-ẹrọ jiini, ọja naa jẹ fermented, ti ṣafihan, ati mimọ ninu Escherichia coli (E. coli), eyiti o dinku viscosity ti supernatant sẹẹli ati iwadii imọ-jinlẹ sẹẹli lysate, ṣugbọn tun mu imudara iwẹnumọ ati iwadii iṣẹ ṣiṣe ti amuaradagba.O tun le ṣee lo ninu itọju Jiini, isọdi-ọlọjẹ ọlọjẹ, iṣelọpọ ajesara, protein ati ile-iṣẹ elegbogi polysaccharide bi isunmọ iyọkuro acid iparun ti ogun.
Awọn ẹya ara ẹrọ ọja
| CAS No. | 9025-65-4 |
| EC No. | |
| Òṣuwọn Molikula | 30kDa |
| Ojuami isoelectric | 6.85 |
| Amuaradagba Mimo | ≥99% (SDS-PAGE & SEC-HPLC) |
| Iṣẹ-ṣiṣe pato | ≥1.1×106U/mg |
| Iwọn otutu to dara julọ | 37°C |
| pH ti o dara julọ | 8.0 |
| Iṣẹ iṣe Protease | odi |
| Ẹru-ẹmi | 10CFU/100,000U |
| Wà Gbalejo-cell Amuaradagba | ≤10ppm |
| Eru Irin | ≤10ppm |
| Endotoxin kokoro arun | 0.25EU/1000U |
| Ifipamọ ipamọ | 20mM Tris-HCl, pH 8.0, 2mM MgCl2, 20mM NaCl, 50% Glycerol |
Awọn ipo ipamọ
≤0°C gbigbe;-25~-15°C Ibi ipamọ,2 years Wiwulo (yago fun didi-thawing).
Unit Definition
Iwọn enzymu ti a lo lati yi iye gbigba ti △A260 nipasẹ 1.0 laarin 30min ni 37 °C, pH 8.0, deede si DNA sperm salmon 37μg digested nipasẹ gige sinu oligonucleotides, ni asọye bi ẹyọ ti nṣiṣe lọwọ (U).
Iṣakoso didara
Wà Gbalejo-cell Amuaradagba: ELISA ohun elo
•Proteate Awọn iyokù: 250KU/mL UltraNuclease ṣe atunṣe pẹlu sobusitireti fun 60min, ko si iṣẹ ṣiṣe ti a rii.
•Endotoxin kokoro arun: LAL-Igbeyewo, Pharmacopoeia ti awọn eniyan Republic of China Iwọn didun 4 (2020 Edition) jeli iye igbeyewo ọna.Awọn ofin gbogbogbo (1143).
•Ẹrù Ẹ̀jẹ̀ Pharmacopoeia ti Orilẹ-ede Eniyan ti Ilu China Iwọn didun 4 (Ẹya 2020) — Gbogbogbo
Ofin fun ailesabiyamo igbeyewo (1101), PRC National Standard, GB 4789.2-2016.
•Irin Eru:ICP-AES, HJ776-2015.
Isẹ
Iṣẹ ṣiṣe UltraNuclease jẹ idinamọ ni pataki nigbati ifọkansi SDS ti kọja 0.1% tabi EDTA
ifọkansi ti kọja 1mM.Surfactant Triton X-100, Tween 20 ati Tween 80 ko ni ipa lori iparun.
Awọn ohun-ini nigbati ifọkansi ba wa labẹ 1.5%.
| Isẹ | Isẹ ti o dara julọ | Isẹ to wulo |
| Iwọn otutu | 37 ℃ | 0-45 ℃ |
| pH | 8.0-9.2 | 6.0- 11.0 |
| Mg2+ | 1-2mM | 1-15mM |
| DTT | 0-100mM | > 100mM |
| 2-Mercaptoethanol | 0-100mM | > 100mM |
| Monovalent irin dẹlẹ (Na+, K+ ati be be lo) | 0-20mM | 0-200mM |
| PO43- | 0-10mM | 0-100mM |
Lilo ati doseji
Yọ acid nucleic exogenous kuro ninu awọn ọja ajesara, dinku eewu ti majele acid nucleic ati ilọsiwaju aabo ọja.
• Din iki ti omi ifunni ti o ṣẹlẹ nipasẹ acid nucleic, kuru akoko ṣiṣe ati mu ikore amuaradagba pọ si.
Yọ acid nucleic kuro eyiti o jẹ patiku ti a we (kokoro, ara ifisi, ati bẹbẹ lọ), eyiti o jẹ iwunilori.
si awọn Tu ati ìwẹnu ti patiku.
| Iru idanwo | Amuaradagba iṣelọpọ | Kokoro, ajesara | Awọn oogun sẹẹli |
| Nọmba awọn sẹẹli | 1g sẹẹli tutu iwuwo (ti tun daduro pẹlu ifipamọ milimita 10) | 1L bakteria olomi supernatant | 1L asa |
| Iwọn to kere julọ | 250U | 100U | 100U |
| Niyanju doseji | 2500U | 25000U | 5000U |
• Itọju iparun le ṣe ilọsiwaju ipinnu ati imularada ti ayẹwo fun chromatography ọwọn, electrophoresis ati itupalẹ blotting.
• Ninu itọju ailera apilẹṣẹ, a ti yọ acid nucleic kuro lati gba awọn ọlọjẹ adeno ti a sọ di mimọ.
