BsaI Ihamọ endonuclease
Apejuwe
Enzymu ihamọ BsaI jẹ iru II ihamọ endonuclease ti o wa lati inu amuaradagba isọdọtun ti a ṣe koodu nipasẹ jiini BspQI ni Bacillus sphaericus ti a fihan nipasẹ E.coli.Awọn endonucleases ihamọ jẹ lilo pupọ ni maapu jiini, cloning ati awọn aaye miiran.Enzymu yii yarayara ge DNA fun laini jiini daradara Bsa I , ihamọ IIs endonuclease ihamọ endonuclease, ti wa lati inu igara E. coli ti o tun ṣe ti o gbe cloned ati iyipada BsaI pupọ lati Bacillus stearothermophilus.eyi ti o ni pato kanna bi awọn enzymu abinibi ati iṣẹ-ṣiṣe irawọ ti o dinku.Ọkọọkan idanimọ rẹ ati awọn aaye fifọ jẹ bi atẹle:
5'·······GGTCTC(N)·········3'
3'······CCAGAG(NNNNN)·······5'
Kemikali Be
Awọn ẹya ara ẹrọ ọja
• Ihamọ endonuclease ni iṣẹ-ṣiṣe enzymatic ti o ga julọ ati pe o pari iṣeduro tito nkan lẹsẹsẹ.
• Kekere ti kii-kan pato iṣẹ endonuclease lati rii daju kongẹ "scalpel" gige.
• Ko si eto BSA, idinku idoti orisun ooru.
Sipesifikesonu
| Awọn nkan Idanwo | Awọn pato |
| Iṣẹ iṣe enzymu | 20 U/µL |
| Mimo(SDS-iwe) | ≥95% |
| Rnase | Ko si ọkan ti a rii |
| Iṣẹ-ṣiṣe DNA ti kii ṣe pato | Ko ṣe awari |
| Star aṣayan iṣẹ-ṣiṣe | Ko ṣe awari |
| Ligation ati Recutting | Lẹhin tito nkan lẹsẹsẹ, awọn ajẹkù DNA le jẹ ligated.Ati pe awọn ajẹkù ligated wọnyi le tun ge pẹlu Bsal. |
| Endotoxins (idanwo LAL) | ≤10 EU/mg |
| E.Coli DNA | ≤1 Daakọ/2U |
Iṣakoso didara
Purity ≥ 95%, Ko si DNase, RNase aṣayan iṣẹ-ṣiṣe, Alejo DNA aloku ≤ 100pg/mg, ogun aloku amuaradagba ≤ -50ppm, iyoku endotoxin ≤ 10EU/mg, Ko si protease aṣayan iṣẹ-ṣiṣe, sterile, free of mycoplasma
Ohun elo
Dije awọn plasmid lati mura a linearized ajeku DNA ni opin ti Poly (A/T/C/G);
Linearize plasmid awoṣe ṣaaju ki o to in vitro transcription;
Daijesti ihamọ;
Unit Definition
Ẹyọ kan jẹ asọye bi iye henensiamu ti o nilo lati da 1 μg ti DNA iṣakoso inu ni wakati 1 ni 37°C ni iwọn ifapa lapapọ ti 50 µL
Gbigbe ati ibi ipamọ
Gbigbe:Ti firanṣẹ labẹ -15 ° C
Ibi ipamọ:Fipamọ ni -25 ~ -15 ° C
Atunyẹwo Igbesi aye niyanju:1 odun
