Deoxyribonuclease I (Dnase I)
Apejuwe
DNase I (Deoxyribonuclease I) jẹ ẹya endodeoxyribonuclease ti o le gbin DNA ọkan- tabi ilopo meji.O mọ ati cleaves phosphodiester bonds lati gbe awọn monodeoxynucleotides tabi nikan- tabi ilopo-strand oligodeoxynucleotides pẹlu fosifeti awọn ẹgbẹ ni 5'-terminal ati hydroxyl ni 3'-terminal.Iṣẹ-ṣiṣe ti DNase I da lori Ca 2+ ati pe o le muu ṣiṣẹ nipasẹ awọn ions irin divalent gẹgẹbi Mn 2+ ati Zn 2+ .5 mM Ca 2+ ṣe aabo fun enzymu lati hydrolysis.Ni iwaju Mg 2+, enzymu le ṣe idanimọ laileto ati ki o ya aaye eyikeyi lori eyikeyi okun ti DNA.Ni iwaju Mn 2+, awọn okun meji ti DNA le jẹ idanimọ nigbakanna ati fifọ ni fere aaye kanna lati ṣe awọn ajẹkù DNA opin alapin tabi awọn ajẹkù DNA opin alalepo pẹlu 1-2 nucleotides ti n jade.
Kemikali Be
Unit Definition
Ẹyọ kan jẹ asọye bi iye henensiamu eyiti yoo sọ di 1 μg ti pBR322 DNA patapata ni iṣẹju mẹwa 10 ni 37°C.
Sipesifikesonu
| Awọn nkan Idanwo | Awọn pato |
| Mimo (SDS-iwe) | 95% |
| Rnase aṣayan iṣẹ-ṣiṣe | Ko si Ibajẹ |
| gDNA Kokoro | ≤ 1 ẹda/μL |
Gbigbe ati ibi ipamọ
Gbigbe:Ti firanṣẹ labẹ 0 °C
Ibi ipamọ:Fipamọ ni -25 ~ -15 ° C
Atunyẹwo Igbesi aye niyanju:2 odun
