D-Lactate dehydrogenase (LDH)
Apejuwe
Enzymu naa ni a lo fun yiyọkuro pyruvate ni awọn ipinnu ṣiṣẹ pẹlu NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
Lilo
1.Alkaline phosphatase pọ si awọn ọlọjẹ (awọn egboogi, streptavidin ati be be lo,) le ṣe idanimọ awọn ohun elo ti o wa ni pato, ati pe o le ṣee lo ni ELISA, WB ati wiwa histochemical;
2.Alkaline phosphatase le ṣee lo lati dephosphorize awọn 5 '-terminal ti DNA tabi RNA lati se ara-sisopọ;
3.Dephosphorylated DNA tabi RNA ti o wa loke le jẹ aami nipasẹ awọn fosifeti ti o ni aami redio (nipasẹ T4 poly-nucleotide kinase)
Kemikali Be
Ilana ifaseyin
D-Lactate + NAD+→ Pyruvate +NADH + H+
Sipesifikesonu
| Awọn nkan Idanwo | Awọn pato |
| Apejuwe | Lulú amorphous funfun, lyophilized |
| Iṣẹ-ṣiṣe | ≥200U/mg |
| Mimo(SDS-PAGE) | ≥90% |
| Solubility (10mg lulú/ml) | Ko o |
| NADH/NADPH oxidase | ≤0.01% |
| Malate dehydrogenase | ≤0.005% |
| Glutamate dehydrogenase | ≤0.003% |
| Pyruvate kinase | ≤0.03% |
| Glutamate Dehydrogenase | ≤0.003% |
| Aspartate aminotransferase | ≤0.001% |
| Alamine aminotransferase | ≤0.001% |
Gbigbe ati ibi ipamọ
Gbigbe:Ambient
Ibi ipamọ:Tọju ni -20°C(igba pipẹ), 2-8°C (akoko kukuru)
Niyanju tun-idanwoIgbesi aye:2 odun
jẹmọ awọn ọja
Kọ ifiranṣẹ rẹ nibi ki o si fi si wa
