Glycerol Kinase (GK)
Apejuwe
Awọn amuaradagba ti a fi koodu si nipasẹ jiini yii jẹ ti idile FGGY kinase.Amuaradagba yii jẹ enzymu bọtini ni ilana ti gbigba glycerol ati iṣelọpọ agbara.O ṣe itọsi phosphorylation ti glycerol nipasẹ ATP, ti nso ADP ati glycerol-3-phosphate.Awọn iyipada ninu jiini yii ni nkan ṣe pẹlu aipe glycerol kinase (GKD).Omiiran spliced tiransikiripiti aba ti o yatọ si awọn isoforms ti a ti ri fun pupọ yi.
Enzymu yii jẹ lilo fun awọn idanwo iwadii fun ipinnu awọn triglycerides papọ pẹlu Glycerol-3-phosphate Oxidase.
Kemikali Be
Ilana Ifa
Glycerol + ATP → Glycerol -3- fosifeti + ADP
Sipesifikesonu
Awọn nkan Idanwo | Awọn pato |
Apejuwe | Funfun to die-die yellowish amorphous lulú, lyophilized |
Iṣẹ-ṣiṣe | ≥15U/mg |
Mimo(SDS-PAGE) | ≥90% |
Solubility (10mg lulú / milimita) | Ko o |
Catalase | ≤0.001% |
Glukosi oxidase | ≤0.01% |
Uricase | ≤0.01% |
ATPase | ≤0.005% |
Hexokinase | ≤0.01% |
Gbigbe ati ibi ipamọ
Gbigbe:Ti firanṣẹ labẹ -15 ° C
Ibi ipamọ:Itaja ni -20°C(igba pipẹ), 2-8°C(igba kukuru)
Niyanju tun-idanwoIgbesi aye:18 osu
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